Data collection methods in SZ-working age
| Measurements | Variables |
| Standardised questionnaire survey | |
| Demographics | National ID, name, date of birth, age, gender, ethnicity, marital status, education level, occupation, income level, type of residence and family structure |
| Lifestyle | Eating habits, physical activity level, smoking status, drinking habits, BMI, recreational activities and time spent using electronic devices |
| Sleep habits | Sleep duration, bedtime, sleep interruptions, early awakening and daytime fatigue |
| Environmental exposure | Air quality, noise exposure, living conditions and exposure to secondhand smoke |
| Work patterns | Working hours, intensity, environment, posture, location and career development |
| Occupational disease exposure history | Dust, radiation, chemical exposure, physical hazards, toxic substances, biological hazards and repetitive actions leading to musculoskeletal pressure |
| Occupational exhaustion | Workload, job autonomy, WLB, occupational exhaustion and job satisfaction |
| Health awareness | Frequency of regular health checkups, vaccination status and health knowledge level |
| Disease management | Chronic disease management, accessibility of medical services and health insurance |
| Systemic disease history | Hypertension, diabetes, coronary heart disease, chronic obstructive pulmonary disease, liver disease, nervous system diseases and autoimmune diseases |
| History of eye diseases | Myopia, hyperopia, astigmatism, dry eye, cataract, glaucoma, macular degeneration, retinal detachment, diabetic retinopathy and pathological myopia |
| Family history | Hypertension, diabetes, coronary heart disease, cerebrovascular disease, cancer, mental illness, Alzheimer’s disease, Parkinson’s disease and hereditary eye diseases |
| Drug allergy history | Allergies to penicillin, sulfa drugs, cephalosporins, aminoglycosides, tetracyclines, anaesthetics, NSAIDs and vaccine components |
| Others | Surgical history, trauma history, sedentary behaviour and self-assessed health status |
| Standardised scale assessments | |
| ADL | Lawton IADL scale |
| Cognitive function | MMSE, MoCA |
| Depression and anxiety | BDI, GAD-7 |
| Quality of life | SF-36 |
| Sleep quality | PSQI, ISI |
| Work stress | PSS, JCQ |
| Occupational burnout | MBI |
| Visual function | NEI VFQ, VF questionnaire |
| Visual fatigue | CVS-Q |
| WLB | WLB scale |
| Social support assessment | Duke-UNC FSSQ, MSPSS |
| Basic ophthalmic examination | |
| Routine examination | Uncorrected visual acuity, corrected visual acuity and intraocular pressure |
| Refractive error | Myopic, hyperopic, astigmatism, astigmatic axis and anisometropia |
| Optical biometry | Corneal curvature radius (horizontal and vertical), central corneal thickness, anterior chamber depth, lens thickness and axial length |
| Tear secretion measurement | Tear secretion volume, tear film breakup time, degree of conjunctival hyperaemia and dry eye score |
| Slit-lamp examination | Corneal epithelial integrity, KP, anterior chamber flare and cell count, lens transparency and vitreous opacities |
| Eye movement | Eye movements, nystagmus, strabismus angle, binocular fusion ability and diplopia |
| Colour vision examination | Type of colour vision deficiency (red–green colour blindness, blue–yellow colour blindness and total colour blindness), colour vision fatigue test and colour vision recovery time |
| Others | Night vision, glare sensitivity, stereopsis test and blink response time |
| Ophthalmic imaging examination | |
| OCT | Macular central foveal thickness, retinal nerve fibre layer thickness, macular retinal thickness, retinal layer structure, optic disc cupping depth and optic disc structure |
| OCTA | Retinal blood flow density, macular blood flow density, optic disc blood flow density, non-perfusion area size, microaneurysms, neovascularisation, choroidal blood flow density, vascular complexity index and retinal vascular distribution |
| Fundus photography | Optic disc colour and morphology, retinal vascular morphology, macular morphology, cup-to-disc ratio, arteriovenous crossing and macular central foveal reflex |
| UWF imaging | Retinal periphery condition, retinal lesion distribution, retinal vascular morphology, retinal pigment changes, peripheral retinal haemorrhages and retinal tears or breaks |
| Anterior segment OCT | Corneal epithelial thickness, anterior chamber depth, anterior chamber angle structure, anterior surface curvature of the lens, anterior chamber angle and lens structure |
| Corneal topography | Central corneal curvature, corneal thickness map, corneal shape symmetry, corneal surface smoothness and corneal morphology |
| Visual field examination | Type of visual field defect, contrast sensitivity, light sensitivity threshold, visual field indices, central visual field clarity and peripheral visual field integrity |
| Biomarker testing | |
| Metabolic function | Fasting blood glucose, glycated haemoglobin, total cholesterol, low-density lipoprotein cholesterol, high-density lipoprotein cholesterol, triglycerides and insulin |
| Liver and kidney function | Alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, total bilirubin, direct bilirubin, albumin, globulin, albumin–globulin ratio, gamma-glutamyl transferase, lactate dehydrogenase, blood urea nitrogen, creatinine and uric acid |
| Vitamins/micronutrients | 25-hydroxyvitamin D, cobalamin and ferritin |
| Inflammatory/immune | C reactive protein, high-sensitivity C reactive protein and homocysteine |
| Tumour biomarkers | Alpha-fetoprotein, carcinoembryonic antigen and carbohydrate antigen 19–9 |
ADL, activities of daily living; BDI, beck depression inventory; BMI, body mass index; CVS-Q, computer vision syndrome questionnaire; FSSQ, functional social support questionnaire; GAD-7, generalised anxiety disorder; IADL, instrumental activities of daily living; ISI, insomnia severity index; JCQ, job content questionnaire; KP, keratic precipitates; MBI, Maslach burnout inventory; MMSE, mini-mental state examination; MoCA, Montreal cognitive assessment; MSPSS, multidimensional scale of perceived social support; NEI VFQ, National Eye Institute Visual Function Questionnaire; NSAIDs, non-steroidal anti-inflammatory drugs; OCT, optical coherence tomography; OCTA, optical coherence tomography angiography; PSQI, Pittsburgh sleep quality index; PSS, perceived stress scale; SF-36, 36-item short form survey; SZ-working age, Shenzhen-working age; UWF, ultrawidefield; VF, visual function; WLB, work-life balance; WLB, work-life balance.