Table 3

Biospecimen collection including processing and purpose

Specimen collection	Sample type	Location	Processing details	Purpose
EDTA vacutainers	Whole blood	Local clinical lab	None	Complete blood count analysis
	Whole blood	UW NORC*	None	Testing for HbA1c
	Plasma	UW NORC*	Processed locally at data site on the day of collection	Testing for NT-proBNP, troponin-T, C-peptide and insulin
	Plasma	Biobanking at UAB CCTS	Processed locally at data site on the day of collection	Future proteomics and metabolomics studies
	Buffy coats	Biobanking at UAB CCTS	Processed locally at data site on the day of collection	DNA extractions for future genomics
Serum separator vacutainers	Serum	Biobanking at UAB CCTS	Processed locally at data site on the day of collection	Future proteomics and metabolomics studies
	Serum	UW NORC*	Processed locally at data site on the day of collection	Testing for CRP-HS, glucose, BUN, creatinine, carbon dioxide, total protein, albumin, globulin, bilirubin, alkaline phosphatase, AST, ALT, electrolytes and lipids
Urine collection kit	Urine	UW NORC*	Processed locally at data site on the day of collection	Testing for creatinine and albumin
CPT mononuclear cell preparation tubes	Peripheral blood mononuclear cells	Biobanking at UAB CCTS	UAB—processed locally on day of collection UCSD/UW—tubes sent to UAB for next-day processing	Future immunological studies; generation of iPSCs
PAXgene RNA vacutainers	Stabilised, unisolated RNA	Biobanking at UAB CCTS	UAB—processed locally on day of collection UCSD/UW—tubes sent to UAB for next-day processing	Future gene expression studies

*Samples analysed by the UW NORC laboratory (serum, plasma, whole blood and urine) are first stored locally at UAB and UCSD at −70°C, then batch-shipped to UW on dry ice approximately once per quarter.
ALT, alanine aminotransferase; AST, aspartate aminotransferase; BUN, blood urea nitrogen; CRP-HS, C reactive protein, high sensitivity; Electrolytes, sodium, potassium, chloride, calcium; Lipid analysis, total cholesterol, high-density lipoprotein cholesterol, low-density lipoprotein cholesterol, triglycerides; NT-proBNP, N-terminal pro-B-type natriuretic peptide; UAB, University of Alabama at Birmingham; UAB CCTS, University of Alabama at Birmingham Centre for Clinical and Translational Science; UCSD, University of California, San Diego; UW, University of Washington; UW NORC, University of Washington Nutrition Obesity Research Center.