Sample storage and processing for the Schista study
Sample | Location | Storage | Sample processing | Location of processing |
Self-collected swab Provider-collected swab | Home | Room temperature (Stored in Primestore media) | Sh DNA (PCR, RPA) GeneXpert- High Risk HPV DNA* | Zambart (Zambia) |
Urine for microscopy | Home | Room temperature | Sh eggs Urine haemastix | District Laboratory (Kafue, Livingstone, Zambia) |
Urine stored for CAA | Clinic | 4°C | Circulating Anodic Antigen | Leiden University Medical Centre (The Netherlands) |
Cervicovaginal lavage | Clinic | −20°C | Sh DNA (PCR, RPA) GeneXpert- CT/NG Microbiota | Zambart (Zambia) |
Blood for serology | Clinic | −20°C | Sh, STI antibody | Zambart (Zambia) |
Colposcopy images | Clinic | Encrypted server | Expert review computer analysis | Zambia London School of Hygien and Tropical Medicine (UK) |
*High-risk HPV by self-collected swab only.
CAA, circulating anodic antigen; HPV, human papillomavirus; RPA, recombinase polymerase assay; Sh, Schistosoma haematobium; STIs, sexually transmitted infections.